Research on technological process of two-pot countercurrent extraction of hydroxysafflor yellow A / 中国中药杂志

<p><b>OBJECTIVE</b>To study the optimum technical conditions of extracting Hydroxysafflor yellow A (HSYA) from Carthmus tinctorius by multi-stage countercurrent extraction technology.</p><p><b>METHOD</b>The effects of extraction time of each stage, extraction temperature, ethanol concentration and solid-liquid ratio (g x mL(-1)) on extraction yield of HSYA were studied by orthogonal test design and the comparison of other extraction methods were presented.</p><p><b>RESULT</b>Extraction time and solid-liquid ratio had significant influence on the extraction yield, and the optimum parameters were as follows: Extraction time of each stage was 120 min, solid-liquid ratio was 1 : 10 (g x mL(-1)), ethanol concentration was 30%, and extracted at room temperature. Under the optimum conditions, the extraction yield of HSYA was 1.56% and the purity of the extract was 6.06%. Compared with the traditional extraction method and the ultrasonic extraction method of the pharmacopoeia, the extraction yield was increased by 6.12% and 9.09%, the purity of extract was increased by 42.9% and 27.0%, respectively.</p><p><b>CONCLUSION</b>The multi-stage countercurrent extraction technology has many advantages such as simple operation, less solvent consumption, higher extraction yield and purity of extract and it has wide industrial application prospect.</p>

Study on fast quality control in extracting process of Paeonia lactiflora using near infrared spectroscopy / 中国中药杂志

<p><b>OBJECTIVE</b>To set up a novel method for fast analysis of active components in water extracting process of Paeonia lactiflora with near infrared spectroscopy (NIRS).</p><p><b>METHOD</b>HPLC was used as the reference method to determine the content of Paeoniflorin and a multivariate calibration model based on PLS algorithm was developed to analyze the correlation between the spectra and the corresponding values determined by the reference method.</p><p><b>RESULT</b>The correlation coefficient of the calibration model was 0.996 2, and the predicted coefficient was 0.989 5. The RMSEC and RMSEP were 0.109 g x L(-1) and 0.138 g x L(-1), respectively, and the RSEP was 5.6%.</p><p><b>CONCLUSION</b>The method mentioned above is proved to be convenient, rapid and nondestructive, accurate and reliable, and is applicable for fast analysis and monitoring of active components in extraction process of traditional Chinese medicine.</p>

Determination of tricin in malt by HPLC / 中草药

Objective To develop an HPLC method for the quantitative analysis of tricin in malt. Methods HPLC method was used. Analysis was performed on Spherigel C_ 18 column (150 mm?4.6 mm, 5 ?m) with acetonitrile-water-tetrahydrofuran-methanoic acid (26∶74∶10∶1) as mobile phase. The detection wavelength was 350 nm, flow rate was 1.0 mL/min, and the analysic volumn was set at 20 ?L. Results The calibration curve of tricin was linear in the range of 0.22-2.15 mg/L (r=0.999 8). The average recovery rate of tricin was 97.4% (RSD=1.7%) (n=9). Conclusion The method can be used to determine tricin in raw malt quantitatively.